Marseille - France
At GENXMAP, we understand that high-quality nucleic acid extraction and purification are the foundation of successful genomic and transcriptomic analysis. Our specialized services cater to a wide range of sample types, ensuring that you receive the highest yield and purity for your research or clinical applications.
DNA Extraction and Purification Expertise Across Diverse Sample Types:
Different samples require different extraction approaches. Our team has developed and optimized protocols to tackle the unique challenges presented by various sample types:
- FFPE Tissues: We employ advanced techniques to extract high-quality DNA from formalin-fixed, paraffin-embedded tissues, overcoming the issues of fragmentation and cross-linking.
- Soil Samples: Our methods effectively remove contaminants such as humic substances, ensuring that you receive clean, inhibitor-free DNA, suitable for even the most sensitive downstream applications.
- Microbiota Samples: With the diversity of microorganisms in microbiota samples, our protocols ensure thorough lysis of all cell types, providing comprehensive DNA extraction for accurate microbial analysis.
- Bone and Teeth Samples: Extracting DNA from bones and teeth can be particularly challenging due to the degradation over time and the presence of calcium-rich matrices. We utilize specialized demineralization and extraction techniques to yield high-quality DNA from these tough tissues.
- Environmental Samples (e.g., water, sediment): Environmental samples often contain low amounts of nucleic acids and various contaminants. Our protocols are designed to isolate DNA even from challenging matrices like sediment and water, ensuring precise and reliable results.
- Plant Materials: Tough plant cell walls and the presence of secondary metabolites can complicate DNA extraction. Our tailored approaches efficiently break down these barriers, ensuring the purity and integrity of DNA from various plant species.
- Formalin-Fixed biological Fluids: Like FFPE tissues, formalin-fixed biological fluids can present difficulties in obtaining high-quality DNA due to cross-linking. We have developed protocols that successfully extract usable DNA from these challenging samples.
- Human and Animal Tissues, Body Fluids, and More: We offer tailored solutions for a wide range of biological samples, guaranteeing optimal results.
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Tailored Solutions: We adapt our extraction processes to your specific sample type, ensuring maximum yield and quality.
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Rigorous Quality Control: Every nucleic acid sample undergoes stringent quality checks before proceeding to downstream applications, ensuring reliability and consistency.
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Advanced Techniques: Our constantly evolving protocols are designed to overcome the toughest challenges in nucleic acid extraction, giving you the confidence to move forward with your research.
Our Quality Control Process
Our quality control services cover every critical stage, from nucleic acid extraction to library preparation for sequencing. We utilize the most advanced tools and techniques to guarantee that your samples are ready for downstream applications.
1. Concentration Measurement
- Qubit Fluorometer: Provides precise quantification of DNA and RNA, ensuring accurate concentration measurements, particularly for low-quantity samples.
- Nanodrop Spectrophotometer: Offers quick concentration measurements along with absorbance-based purity ratios.
2. Purity Assessment
- Nanodrop Spectrophotometer: Analyzes 260/280 and 260/230 ratios to identify protein contamination and organic impurities.
- Agarose Gel Electrophoresis: Visualizes nucleic acids to detect contaminants and assess sample purity
3. Integrity and Fragment Size Analysis
- Fragment Analyzer (Qiaxcel Connect), Bioanalyzer, and TapeStation: These instruments provide detailed electrophoresis-based profiles of RNA and DNA integrity, including RNA Integrity Number (RIN) and DNA Integrity Number (DIN) patterns.
4. PCR-Based Quality Control
- qPCR/dPCR: Assesses the amplification efficiency of DNA or RNA, ensuring suitability for sensitive downstream applications.
- RT-qPCR: Validates RNA extraction quality by quantifying specific transcripts, crucial for transcriptomic studies.
5. Agarose Gel Electrophoresis
- Gel Visualization: Offers a straightforward method to detect degradation and contamination in DNA or RNA samples.
6. UV-Vis Spectrophotometry
- Absorbance Ratios: Complements other purity assessments, providing an overview of nucleic acid quality.
7. Capillary Electrophoresis
- CE-Based Analysis: Delivers high-resolution separation to confirm the integrity and precise sizing of nucleic acid fragments.
Ready to optimize your nucleic acid extraction and quality process? Contact us today to learn how our services can support your research and clinical needs.
