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ELISA(enzyme-linkedimmunosorbent assay)

Protein analysis through proteomics is essential for a comprehensive understanding of biological function, complementing genomic and transcriptomic data. It enables the functional validation of the expression and secretion of key biomarkers, ensuring that the molecular changes observed at the gene and RNA levels are reflected in actual protein activity.

 

GENXMAP offers a range of advanced proteomic techniques, including:

 

  • Immunohistochemistry/fluorescence (IHC) / Immunocychemistry/fluoresence (ICC)

  • Microscopy imaging analyses (Confocal Microscopy)

  • Flow Cytometry

  • Enzyme-Linked Immunosorbent Assay (ELISA)

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Enzyme-Linked Immunosorbent Assay (ELISA)

 

  • Description: ELISA is a widely used biochemical assay that quantitatively measures the presence of specific proteins, such as antibodies, hormones, or cytokines, in a sample (e.g., blood, urine, or cell lysates). In this technique, the target antigen is immobilized on a solid surface (microplate) and then bound by an antibody linked to a reporter enzyme. Upon adding a substrate, the enzyme catalyzes a reaction that produces a measurable color change, which reflects the concentration of the protein of interest.
     

  • Applications

    • Diagnostic Testing

    • Infectious Disease Detection

    • Hormone Level Measurement

    • Cytokine and Growth Factor Quantification

    • Allergen Detection

    • Antibody Detection

    • Vaccine Development

    • Biomarker Validation

    • Food Safety Testing

    • Environmental Monitoring

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