Biosourcing involves collecting and providing high-quality biological samples essential for research and development. It ensures access to materials tailored to scientific needs, guaranteeing their traceability and relevance for the study of diseases, biomarkers, and therapeutic responses.

RNA extraction involves isolating RNA from biological cells or tissues. High-quality extraction and purification of nucleic acids are crucial to ensure the success of transcriptomic analysis.

A quantitative PCR (qPCR) gene expression test assesses the amount of specific mRNA in a sample. This process amplifies targeted genetic sequences and measures their levels through fluorescence, providing precise information on gene expression activity.

Library preparation converts extracted nucleic acid sequences into a format compatible with sequencing. This process includes fragmentation of the sequences, the addition of adapter sequences, and steps for purification and quality control to ensure the accuracy and precision of the data.

Single-cell RNA sequencing (scRNA-seq) analyzes gene expression at the level of individual cells, providing an in-depth view of the cellular transcriptome. This advanced technology is valuable for exploring tissue complexity, cellular diversity, and dynamic biological processes.

Sequencing determines the exact order of nucleotides (A, T, C, G) in a DNA molecule. To sequence RNA, the RNA is first converted into complementary DNA (cDNA), which is then used to prepare the libraries.

Spatial transcriptomics maps RNA transcripts to precise locations within tissue architecture. By combining histological imaging with high-throughput sequencing, this approach captures gene expression variations across different cell types while preserving their original spatial context.