GENXMAP | RNA Library Preparation Services

Optimize Your RNAseq

Optimize your research with our RNA library preparation service. We convert nucleic acid sequences into formats suited for high-precision sequencing, ensuring reliable and comprehensive results. Leverage our expertise for optimal transcriptomic analysis.

Total RNA sequencing (RNA-seq)

Bulk RNA-seq allows for the quantification of total gene expression or all mRNA from a cell mixture, providing an overview of mRNA levels and transcriptional regulation within a sample. Our bulk RNA sequencing service provides you with the necessary data to advance your research.

Single-cell sequencing

Single-cell RNA sequencing allows the analysis of gene expression at the individual cell level within a given population, simultaneously measuring the expression of hundreds to thousands of genes. This technique unveils the heterogeneity of cell populations, reconstructs cellular development trajectories, and models transcriptional dynamics, providing insights that were previously masked by bulk RNA sequencing methods.

RNA Library Preparation

Transforming the invisible into readable, RNA library preparation, key to exploring the transcriptome.

Accelerate Biomarker Discovery with GENXMAP

Project Study / Quote

GENXMAP offers a library preparation service from RNA, specially designed for total RNA sequencing (bulk) or single-cell RNA sequencing (sc-RNA seq) applications. With this advanced approach, it is possible to accurately quantify RNA levels, identify genes whose expression varies under different conditions, and explore complex processes like alternative splicing. By integrating these solutions into your projects, you gain access to robust and detailed gene expression analyses, whether for large-scale studies or research on individual cells.

GENXMAP ensures a deeper understanding of genetic mechanisms and significant progress in biomedical research.

Pure RNA, Unlocking Precision Analysis

Service Details

Identifying the Objectives

Sample Type: Nature, quantity, condition, and availability of samples (purified RNA, cells, tissues, etc.). Envisioned Technologies: Advice on platform selection (Illumina).

Sequencing Type Selection:

Bulk RNA-seq (on total RNA, mRNA, microRNA)

Single-cell RNA-seq

Defining Parameters:

Sequencing depth

Genomic coverage

Multiplexing (if applicable)

Resource Validation:

Evaluation of the quality and quantity of available samples.

Advice on upstream preparation steps: extraction, purification, quality control.

Defining the Budget:

Discussion of fixed or flexible budget.

Establishing the Timeline:

Estimation of timeframes for each stage.

Planning deliverables: raw data, analysis, reports.

At the end of this consultation, you will receive:

A detailed proposal: methods, technologies, budget, and timeline.

A personalized strategy to maximize the success of your project.

Clear guidance on how to prepare your samples and optimize results.
High-throughput sequencing (Next-Generation Sequencing - NGS): GENXMAP uses the following platforms:

NovaSeq (Illumina)

Advantages:

Fast and accurate sequencing of large amounts of DNA from various environmental or biological sources.

Provides high coverage and depth for precise detection of microorganisms present.

MiSeq (Illumina)

Advantages:

High accuracy: Illumina's synthesis sequencing technology is known for its low error rate, making it ideal for analyses that require high precision, such as genetic variation studies.

Rapid turnaround: Quick run times enable timely results for urgent projects.

Oxford Nanopore Technology

Advantages:

Long reads: ONT allows the generation of very long reads (up to several hundred kb), which is valuable for studying complex genomic regions, whole genomes, and full transcripts without fragmentation.

Real-time data: Immediate analysis as sequencing occurs, providing instant insights.
Step 0 >> Sample Receipt Control

Visual evaluation of quantity and quality.

Report and client decision "Go / No-Go" for proceeding with the process.

Step 1: Sample Preparation

DNA/RNA Extraction: Optimized protocols ensure high-quality extraction, even from challenging samples (FFPE tissues, rare cells, etc.).

Quantification and Quality Assessment: Verification of DNA/RNA quantity and quality using spectrophotometers (Nanodrop), Qubit, or Fragment Analyzer (Qiaxcel).

Optional: mRNA enrichment via: i) ribosomal RNA removal, ii) polyA mRNA selection.

For Single-Cell RNA Sequencing: Please refer to the specific page for the technique and process.

Library Preparation: After DNA is extracted and optionally enriched, it is fragmented, and the necessary adapters for sequencing are added, creating a sequencing library ready for analysis on the chosen platform.

Step 2 >> Sequencing Launch

Based on the selected technology (Illumina), sequencing is performed on the appropriate platform.

Step 3 >> Real-Time Quality Control

Monitoring of the sequencing process to ensure the absence of technical errors and maintain the quality of the generated data.

Step 4 >> Data Analysis

We offer different levels of analysis: primary, secondary, or tertiary, depending on your needs. We collaborate with our partners to provide various types of analyses.

For more details, please visit our "Bioinformatics & Biostatistics Analysis" page.

Step 5 >> Validation and Interpretation

Data Interpretation: Our bioinformatics experts and partners analyze the results to provide an in-depth interpretation. This analysis helps uncover complex relationships between microorganisms and identify potential biomarkers or therapeutic targets.

Detailed Reports: A comprehensive report is generated, including result visualizations (GO pathways, UMAP, etc.), conclusions, and recommendations for the next steps based on project objectives.

Step 6 >> Results Delivery

Result Formats: Raw data files (FASTQ) provided.

Detailed Report: Includes a summary of results, visualizations (based on requested analysis), biological interpretation, and recommendations for next steps.

Delivery Methods: Access via a secure online platform, sent via email, or through a results presentation meeting.

Follow-up and Support: Post-delivery assistance for clarifications, revisions, or additional analyses.

Delivery Timeline: Typically between 3 to 6 weeks, depending on project complexity.