Types of Analyzed Biological Materials
We offer RNA extraction services tailored to a wide range of biological materials, ensuring reliable and high-quality results for your analyses.
FFPE samples (Formalin-Fixed, Paraffin-Embedded)
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RNA extraction from FFPE tissues is complex due to cross-linking and degradation. Our optimized protocols maximize the recovery of high-quality, usable RNA.
Blood samples
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Blood contains RNases that can degrade RNA. Our optimized protocols ensure rapid stabilization and effective inhibition to preserve its integrity.
Microbiome samples
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Microbial RNA extraction requires lysis methods tailored to the diversity of microorganisms. Our protocols ensure optimal yield and preserved integrity for accurate analyses.
Plant materials
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Plant RNA extraction is complex due to cell walls and metabolites. Our optimized protocols ensure efficient lysis and high-quality purification for all species.
Soil and Environmental Samples
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RNA extraction from soil and environmental samples is challenging due to inhibitors and low RNA content. Our optimized methods ensure high-quality RNA for sensitive analyses.
Bone and Cartilage
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These samples, with dense matrices and low RNA content, complicate extraction. Our specialized dematerialization techniques optimize the recovery of usable RNA.
Spheroids and Organoids
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Spheroids and organoids, with their dense 3D structure, complicate RNA extraction. Our optimized protocols ensure homogeneous lysis and maximum RNA preservation for accurate analyses.
Tissue and Organ Samples
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Tissues, such as brain or muscle, vary in RNA yield and stability. Our tailored protocols ensure optimal extraction, preserving integrity and quality for each sample.
Fresh Frozen and Fixed Tissues
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Whether frozen or fixed tissues, our protocols minimize degradation and optimize yield, ensuring high-quality RNA for your analyses.
Service Details
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We clearly define your scientific objectives to guide your study, whether it is analyzing specific markers through RT-qPCR or exploring further with analyses such as Bulk RNA-seq or single-cell RNA-seq. Each approach is tailored to your needs to maximize the relevance and accuracy of the results.
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You specify the types of samples you wish to analyze (tissues, cells, environmental samples...), which allows us to adapt the extraction process to ensure the quality and precision of the results.
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Methodology Selection:
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Based on your objectives, we advise you on the most appropriate method for extraction and choosing an exploratory or targeted approach.
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Project Planning and Customization:
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Specific Targeting or Global Sequencing: If necessary, we design a specific panel to target particular genes or groups of interest, or opt for a global approach to analyze genetic diversity as a whole.
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Budget and Timeline Estimation:
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Budget Validation: We provide a clear estimate of the project cost based on the selected methodology, the number of samples, and the chosen analysis technique.
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Timeline Planning: We share an estimate of the time required for sample preparation and transcriptomic analyses (exploratory or targeted), with regular check-ins to track progress.
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Ongoing Monitoring and Support:
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Support throughout the process: Once the project is underway, we support you throughout the entire process, from raw data collection to bioinformatics assistance for interpreting the results, with personalized reports and recommendations for next steps.
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RNA Extraction Methods:
RNA extraction can be performed using: Trizol method, columns, or magnetic beads.
Quantification and Quality Control using the Most Appropriate Method:
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Concentration Measurement:
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Qubit: Accurate DNA/RNA quantification, ideal for low-concentration samples.
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Nanodrop Spectrophotometer: Quick and efficient quantification, measures purity based on absorbance.
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Purity Measurement:
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Nanodrop Spectrophotometer: Measures 260/280 and 260/230 ratios to detect protein or organic contamination.
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Agarose Gel Electrophoresis: Allows visualization of nucleic acids and detection of contaminants.
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Fragment Size and Integrity Analysis:
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Fragment Analyzer (Qiaxcel Connect), Bioanalyzer, and TapeStation: Provide detailed RNA and DNA integrity profiles, including RIN and DIN values.
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Quality Control Based on PCR:
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qPCR/dPCR: Evaluates the amplification efficiency of DNA/RNA.
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RT-qPCR: Verifies RNA extraction quality by quantifying specific transcripts.
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Agarose Gel Electrophoresis:
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Gel Visualization: Simple method for evaluating sample degradation and contamination of DNA/RNA.
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Capillary Electrophoresis (CE):
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CE-based Analysis: High-resolution fragment separation to check nucleic acid integrity and size.
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Analysis Report:
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A comprehensive report including sample concentration and quality.
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At GENXMAP, we understand that nucleic acid extraction and purification are critical steps for the success of any genomic or transcriptomic analysis. We offer specialized services tailored to a wide variety of sample types, including tissues, cells, and biological fluids.
Our expertise ensures optimal yields and impeccable purity—two essential criteria for obtaining reliable and precise results, whether for fundamental research projects or clinical applications. By choosing GENXMAP, you benefit from proven expertise, cutting-edge technologies, and personalized support, ensuring high-quality results for any project.