At GENXMAP, our DNA library preparation service is designed to maximize the potential of your research projects by providing the highest quality samples. With our expertise, we tailor the library preparation process to the type of sample and the specific goals of your study. Whether you need targeted coverage for sequencing studies or a more comprehensive approach, we ensure accurate conversion of nucleic acids into formats compatible with the most advanced sequencing technologies. This customization guarantees reliable and reproducible results, essential for any genomic analysis.
Our service is designed to optimize the quality of the data generated, while adapting to the specific needs of each client. We offer personalized solutions to convert your DNA samples into libraries ready for sequencing.
Examples of library preparations offered by GENXMAP and their applications:
Unlocking the Power of Genomic Data Through Expert Library Preparation and Sequencing
Service Details
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Identification of Objectives
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Global Analysis, Targeted Studies, Clinical Validation, etc.
Define your scientific goals, such as variant detection, gene analysis, or clinical validation, and identify the most appropriate sequencing approach.
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Sample Types
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Nature, Number, State, and Availability
Discuss the type of samples (purified DNA, cells, tissues) and their quality to ensure suitability for your project.
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Technologies Considered
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Platform Consultation:
We provide guidance on selecting the best sequencing platform for your project (Illumina, PacBio, Oxford Nanopore, Sanger, etc.).
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Sequencing Type Selection
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Whole Genome Sequencing (WGS)
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Whole Exome Sequencing (WES)
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RNA-seq (Transcriptomic Profiling)
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Targeted or Metagenomic Sequencing
Choose the most relevant approach based on your research objectives.
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Parameter Definition
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Sequencing Depth, Genomic Coverage, and Multiplexing (if applicable)
Tailor the parameters to meet your desired resolution and coverage for accurate variant detection.
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Resource Validation
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Sample Quality and Quantity Assessment
We evaluate the quality and quantity of available samples to ensure their suitability for the sequencing process. -
Preprocessing Advice:
Guidance on sample preparation steps such as extraction, purification, and quality control.
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Budget Definition
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Fixed or Flexible Budget Discussion
We provide a detailed breakdown of costs based on sequencing parameters and technology choices.
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Timeline Development
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Step-by-Step Estimation:
Provide a timeline for each stage of the project, from sample preparation to data analysis. -
Deliverables Planning:
Define the expected deliverables, including raw data, analyses, and reports.
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Outcome of the Consultation:
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Detailed Proposal: A comprehensive document including methods, technologies, budget, and timeline.
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Personalized Strategy: A clear, customized plan to ensure the success of your project.
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Sample Preparation Guidance: Practical advice on optimizing your samples for sequencing and maximizing data quality.
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Genomic Libraries:
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Whole Genome Sequencing (WGS) Libraries
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Used for comprehensive genome-wide analysis of genetic variations (SNPs, CNVs, etc.).
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Targeted Libraries:
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Targeted Sequencing of Specific Regions or Gene PanelsApplications:
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Study of mutations in genes of interest.
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Clinical validation of genetic variants.
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Metagenomic Libraries:
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For Analyzing Complex Samples Containing Multiple Organisms (Microbiomes, Environmental Samples)
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Compatible with amplification of specific regions (e.g., 16S gene for bacteria).
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Step 0 > Sample Evaluation Upfront:
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Verification of incoming DNA quality to maximize the chances of success.
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Report sent to clients, with a "Go / No-Go" decision for the continuation of the process.
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Step 1 > DNA Fragmentation:
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DNA is fragmented to a specific size to meet the sequencing platform requirements.
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Methods used:
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Enzymatic fragmentation.
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Mechanical fragmentation (e.g., sonication).
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Step 2 > End Repair and Adapter Addition:
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Modification of DNA fragments to create suitable ends.
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Adapter addition for fragment recognition by the sequencing machine.
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Options available:
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Universal or project-specific adapters.
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Barcodes (indexes) for multiplexing multiple samples on the same sequencing lane.
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Step 3 > Enrichment and Amplification:
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PCR amplification to enrich target fragments.
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Validation of amplified fragments to ensure uniformity.
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Step 4 > Library Quality Control:
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Fragment size analysis (e.g., Bioanalyzer, TapeStation).
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Precise quantification (Qubit, qPCR).
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Purity and contamination checks.
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Step 5 > Library Delivery:
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DNA library ready for sequencing.
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Quality control report including:
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Fragment size.
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Total library quantity.
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Purity analysis results.
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Optimizing Protocols:
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GENXMAP adjusts protocols according to project constraints (limited DNA quantity, degraded quality, etc.).
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Custom Library Design:
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Project-specific adapters.
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Indexing for multiplexed sequencing.
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Low-Input Library Preparation:
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Protocols tailored for samples with limited DNA quantity.
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Libraries for Degraded DNA:
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Optimized solutions for FFPE samples or ancient DNA (paleogenetics).
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Automated Preparation:
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Minimizes variability risk for large-scale projects.
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Fast Preparation:
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Ideal for projects with strict time constraints.
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Reduced turnaround time: 3 to 5 business days (depending on project complexity).
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Priority support.
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Process optimization.
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GENXMAP, your trusted partner to support you in your research.
Optimized and Transparent Services
Our Go/No-Go processes allow you to track the progress of your project in real-time. At each stage, you validate the transition to the next, ensuring complete control and maximum transparency throughout the entire process.
Our optimized timelines ensure fast and efficient execution of each project while meeting your specific requirements. This system ensures proactive time management, minimizing delays and maximizing productivity to deliver results that align with your expectations.
Our tailored processes are designed to meet the unique needs of each project, offering flexibility and personalized follow-up. This system ensures rigorous control while delivering accurate results that align with your expectations.
Our detailed analyses guarantee reliable and in-depth results tailored to each study. With cutting-edge tools and proven methodologies, we provide a precise understanding of genetic data, while adhering to the highest standards of quality and scientific rigor.
Prepare your samples optimally for precise results.
Meticulous preparation for optimized libraries > Reliability and precision guaranteed.
DNA library preparation
Objective:
Explore genetic variations, detect mutations, profile microbiomes, analyze gene expression, and diagnose diseases.
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Customized preparation of high-quality DNA libraries compatible with advanced sequencing technologies.
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