Next-generation sequencing technologies provides deep and precise profiling of genome and transcriptome. GENXMAP, expert in sample preparation, proposes the library preparation for sequencing purpose. The sample preparation process varies depending on the sample type and the purpose of the experiment. Different types of genetic material (DNA or RNA) have different sample preparation processes. On top of that, the different applications of NGS add another dimension. Therefore, there are several questions that need to be asked before the experiment to determine the best methods to optimize the most the library preparation process.  The goal of library preparation is to convert the extracted nucleic acids into a format that is appropriate for the sequencing technology fits the most to the purpose of study. 

 

METAGENOMICS APPLICATIONS PROPOSED BY GENXMAP​

   - Description: Sequencing and analyzing the collective genome of microbial communities in environmental samples.

   - Applications: Biodiversity studies, environmental monitoring, and industrial microbiology.

 

i. 16S rRNA Gene Sequencing

• Purpose: To identify and compare bacterial and archaeal communities based on the 16S ribosomal RNA gene, a highly conserved component of the ribosome.

• Applications:

  • Microbial diversity and taxonomy in environmental samples (soil, water, etc.).

  • Gut microbiome analysis.

  • Pathogen detection in clinical samples.

  • Environmental monitoring and bioremediation.

ii. 18S rRNA Gene Sequencing

• Purpose: To study eukaryotic microorganisms (e.g., protists, fungi, algae) by targeting the 18S ribosomal RNA gene.

• Applications:

  • Eukaryotic microbiome studies.

  • Biodiversity assessments in natural and engineered ecosystems.

  • Parasitology and environmental health studies.

iii. Shotgun Metagenomics

• Purpose: To sequence all the DNA present in a sample, providing a comprehensive view of all the organisms present and their functional potential.

• Applications:

  • Comprehensive microbial community profiling, including bacteria, archaea, fungi, viruses, and eukaryotes.

  • Functional genomics and metabolic pathway analysis.

  • Detection of rare or novel organisms.

  • Discovery of antibiotic resistance genes and other functional traits.​

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