At GENXMAP, we understand that high-quality nucleic acid extraction and purification are the foundation of successful genomic and transcriptomic analysis. Our specialized services cater to a wide range of sample types, ensuring that you receive the highest yield and purity for your research or clinical applications. RNA extraction presents unique challenges depending on the sample type, particularly because of RNA's susceptibility to degradation. Our team has developed and optimized protocols to address these difficulties and ensure the highest quality of RNA:

 

​RNA Extraction and Purification Expertise Across Diverse Sample Types:

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- FFPE Tissues: Extracting RNA from formalin-fixed, paraffin-embedded tissues is particularly challenging due to cross-linking and degradation caused by the fixation process. Our advanced extraction protocols are designed to recover the best possible RNA quality from these compromised samples.

 

- Blood Samples: Blood contains ribonucleases (RNases) that can rapidly degrade RNA, making extraction challenging. We utilize optimized procedures that include rapid stabilization and efficient RNase inhibition to preserve RNA integrity during the extraction process.

 

- Microbiota Samples: The diverse and complex nature of microbial communities requires specialized lysis methods to extract RNA from various microorganisms. Our protocols ensure effective RNA extraction across different microbial species, preserving the integrity of the RNA for accurate downstream analysis.

 

- Plant Materials: Plant tissues present challenges due to rigid cell walls and the presence of secondary metabolites that can interfere with RNA extraction. Our tailored protocols effectively break down these barriers and remove contaminants, ensuring high-purity RNA extraction from various plant species.

 

- Soil and Environmental Samples: Extracting RNA from soil and environmental samples is difficult due to low RNA content and the presence of inhibitors. We have developed optimized methods to extract RNA from these challenging matrices, yielding high-quality RNA suitable for sensitive downstream applications.

 

- Bone and Cartilage: These samples are notoriously difficult for RNA extraction due to their dense, mineralized matrices and low RNA content. Our specialized demineralization and extraction techniques allow for the successful recovery of RNA from even these tough tissues.

 

- Spheroids and Organoids: Spheroids and organoids present unique challenges due to their 3D structure and dense cellular composition, which can lead to uneven RNA extraction and increased RNA degradation. Our optimized workflows ensure complete lysis and efficient extraction, preserving the high integrity and quality of RNA needed for accurate downstream analysis.

 

- Tissue and Organ Samples: Different tissues, such as brain or muscle, can vary in RNA yield and susceptibility to degradation. Our workflows are optimized to address the specific needs of each tissue type, ensuring the highest RNA integrity and yield.

 

- Fresh-Frozen and Fixed Tissues: Whether working with fresh-frozen or chemically-fixed tissues, our extraction protocols are designed to minimize RNA degradation and maximize yield, providing high-quality RNA for your downstream applications.

 

Why Choose GENXMAP?

 

- Tailored Solutions: We customize our RNA extraction processes based on the specific challenges posed by each sample type, ensuring the highest yield and purity.

 

- Rigorous Quality Control: Each RNA sample undergoes comprehensive quality checks, including RNA integrity assessment, to guarantee that it meets the necessary standards before moving on to downstream applications.

 

- Advanced Techniques: Our constantly evolving extraction protocols are designed to overcome the most difficult challenges in RNA extraction, providing you with reliable, high-quality RNA for your research.

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Our Quality Control Process

 

Our quality control services cover every critical stage, from nucleic acid extraction to library preparation for sequencing. We utilize the most advanced tools and techniques to guarantee that your samples are ready for downstream applications.

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1. Concentration Measurement

   - Qubit Fluorometer: Provides precise quantification of DNA and RNA, ensuring accurate concentration measurements, particularly for low-quantity samples.

   - Nanodrop Spectrophotometer: Offers quick concentration measurements along with absorbance-based purity ratios.

 

2. Purity Assessment

   - Nanodrop Spectrophotometer: Analyzes 260/280 and 260/230 ratios to identify protein contamination and organic impurities.

   - Agarose Gel Electrophoresis: Visualizes nucleic acids to detect contaminants and assess sample purity

 

3. Integrity and Fragment Size Analysis

 - Fragment Analyzer (Qiaxcel Connect), Bioanalyzer, and TapeStation: These instruments provide detailed electrophoresis-based profiles of RNA and DNA integrity, including RNA Integrity Number (RIN) and DNA Integrity Number  (DIN) patterns.

 

4. PCR-Based Quality Control

   - qPCR/dPCR: Assesses the amplification efficiency of DNA or RNA, ensuring suitability for sensitive downstream applications.

   - RT-qPCR: Validates RNA extraction quality by quantifying specific transcripts, crucial for transcriptomic studies.

 

5. Agarose Gel Electrophoresis

   - Gel Visualization: Offers a straightforward method to detect degradation and contamination in DNA or RNA samples.

 

6. UV-Vis Spectrophotometry

   - Absorbance Ratios: Complements other purity assessments, providing an overview of nucleic acid quality.

 

7. Capillary Electrophoresis

   - CE-Based Analysis: Delivers high-resolution separation to confirm the integrity and precise sizing of nucleic acid fragments.

 

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Ready to optimize your RNA extraction process? Contact us today to learn how our services can support your research and clinical needs.