Next-generation sequencing technologies provides deep and precise profiling of genome and transcriptome. GENXMAP, expert in sample preparation, proposes the library preparation for sequencing purpose. The sample preparation process varies depending on the sample type and the purpose of the experiment. Different types of genetic material (DNA or RNA) have different sample preparation processes. On top of that, the different applications of NGS add another dimension. Therefore, there are several questions that need to be asked before the experiment to determine the best methods to optimize the most the library preparation process.  The goal of library preparation is to convert the extracted nucleic acids into a format that is appropriate for the sequencing technology fits the most to the purpose of study. 

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WHOLE GENOME SEQUENCING (WGS) and Genome-Wide Association Studies (GWAS) APPLICATIONS PROPOSED BY GENXMAP

Whole genome sequencing (WGS) can be performed using various technologies and approaches, each suited to different research needs and applications. Here’s a breakdown of the different types of whole genome sequencing:

 

i. Short-Read Sequencing

 

• Description: Utilizes technologies that generate relatively short DNA sequence reads (typically 100-300 base pairs).

•  Technologies: Illumina (Solexa) Sequencing is currently the most widely used method for short-read sequencing. It provides high-throughput and cost-effective sequencing with high accuracy and is used for a broad range of applications, including variant detection, structural variation analysis, and expression studies.

• Applications:

  • Variant detection (SNPs, small in/dels).

  • Whole genome studies for human, animal, plant, and microbial genomes.

  • Genome assembly and annotation.

 

ii. Long-Read Sequencing

 

• Description: Generates longer DNA sequence reads, which help in resolving complex genomic regions, repetitive sequences, and structural variants.

• Technologies: Oxford Nanopore Technologies (ONT) uses nanopore-based sequencing to provide reads of variable lengths, ranging from thousands to over a million base pairs. It is highly useful for real-time sequencing and long-read applications.

• Applications:

  •    Complex genome assembly and phasing.

  • Structural variant detection.

  • Analysis of repetitive regions and large structural rearrangements.

  • Full-length transcript sequencing.